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School of Medicine Columbia

Light and Live Cell Microscopy

Light microscopy, a cornerstone of scientific exploration, employs visible light to magnify and visualize specimens at the cellular and subcellular levels. By harnessing the principles of optics, this technique allows researchers to uncover the intricacies of biological structures with remarkable clarity and detail, paving the way for breakthroughs in fields ranging from biology and medicine to materials science.

Equipment Options

Details

  • Excitation and emission filters for UV, green, red and far red dyes
  • Fiber optic darkfield illuminator for high-resolution darkfield imaging
  • Wide range of Plan and Plan-Fluor objectives ranging from 4X through 100X oil
  • Phase and DIC objectives and condensers
  • Micropublisher digital camera with Qimaging software

Location

IRF B59

IRF Contacts

Anna Harper

The Nikon Eclipse E800 upright research microscope is designed with a CFI60 optical system, a modern infinity-corrected optical system that allows for long working distances and high numerical apertures. This optical system supports observation methods including: brightfield, epi-fluorescence, Nomarski DIC, phase contrast, polarization, and darkfield. Introduced with the Eclipse E800 was a Macro Plan UW 0.5x magnification objective, which accommodates an exceptionally large 50 mm field of view when looking through the eyepieces.

Location

IRF B59

IRF Contact

Anna Harper

The Discover Echo Revolve is the world’s first and only hybrid, 4-in-1 microscope. Options include:

  • The upright microscope- ideal for glass slides
  • The inverted microscope- ideal for live cell imaging
  • Transmitted light- brightfield, darkfield, phase contrast, and Polarized light
  • Fluorescence- automated LED illumination and one touch image overlay

Using the large touchscreen, users can control camera and illumination functions with their fingertip.

Widefield Fluorescence Images can encounter haze artifacts produced by out of focus light. ECHO’s proprietary Digital Haze Reduction (DHR) software module reveals fine details by suppressing noise and reducing blur to provide stunning, crystal-clear images

Location

IRF B59

IRF Contacts

Anna Harper

The EVOS is a fully automated, digital inverted multi-channel fluorescence and transmitted light imaging system equipped with: 

  • Light cubes: Dapi, GFP, Texas Red and Cy5
  • 4X, 10X phase, 20X, 20X phase, 40X and 60X objectives
  • Vessel holders available: double slide, multiwell, 35mm dish, 60mm dish, 100mm dish, T-25 flask and T-75 flask

Location

IRF B59

IRF Contacts

Lorain Junor or Anna Harper

Equipment Website

Details

  • Filter sets for fluorescent imaging of EGFP, Rhodamine, CFP, YFP, Cy3 and DAPI ranges of dyes
  • LCD cold light source for transmitted light imaging
  • Fiber optic illumination source
  • Zeiss Axiocam MRm digital camera with Axiovision software

Location

IRF B59

IRF Contact

Lorain Junor

 


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